Davis Ng
Alumni Postdoc
Selected Publications
Boone M, Wang L, Lawrence R, Frost A, Walter P, Schoof M. A point mutation in the nucleotide exchange factor eIF2B constitutively activates the integrated stress response by allosteric modulation. eLife 11:e76171, 2022
(PMID: 35416150)
Yildirim Z, Baboo S, Hamid SM, Dogan AE, Tufanli O, Robichaud S, Emerton C, Diedrich JK, Vatandaslar H, Nikolos F, Gu Y, Iwawaki T, Tarling E, Ouimet M, Nelson DL, Yates JR 3rd, Walter P, Erbay E. Intercepting IRE1 kinase-FMRP signaling prevents atherosclerosis progression. EMBO Mol Med 14(4):e15344, 2022
(PMID: 35191199)
Le Thomas A, Ferri E, Marsters S, Harnoss JM, Lawrence DA, Zuazo-Gaztelu I, Modrusan Z, Chan S, Solon M, Chalouni C, Li W, Koeppen H, Rudolph J, Wang W, Wu TD, Walter P, Ashkenazi A. Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α. Nat Comms DOI: 10.1038/s41467-021-27597-7, 2021
(PMID: not available)
Schoof M, Wang L, Cogan JZ, Lawrence RE, Boone M, Wuerth JD, Frost A, Walter P. Viral evasion of the integrated stress response through antagonism of eIF2-P binding to eIF2B. Nat Comms (2021) 12:7103, 2021
(PMID: not available)
Wang L, Toutkoushian H, Belyy V, Kokontis C, Walter P. Conserved structural elements specialize ATAD1 as a membrane protein extraction machine. bioRxiv DOI: 10.1101/2021.09.24.461712, 2021
(PMID: not available)
Schoof M, Wang L, Cogan JZ, Lawrence R, Boone M, Wuerth JD, Frost A, Walter P. Viral Evasion of the Integrated Stress Response Through Antagonistic eIF2-P Mimicry. bioRxiv doi: https://doi.org/10.1101/2021.06.07.447473, 2021
(PMID: not available)
Schoof M, Boone M, Wang L, Lawrence R, Frost A, Walter P. eIF2B conformation and assembly state regulate the integrated stress response. eLife 10:e65703, 2021
(PMID: 33688831)
Schoof M, Faust B, Saunders RA, Sangwan S, Rezelj V, Hoppe N, Boone M, Billesbølle CB, Puchades C, Azumaya CM, Kratochvil HT, Zimanyi M, Deshpande I, Liang J, Dickinson S, Nguyen HC, Chio CM, Merz GE, Thompson MC, Diwanji D, Schaefer K, Anand AA, Dobzinski N, Zha BS, Simoneau CR, Leon K, White KM, Chio US, Gupta M, Jin M, Li F, Liu Y, Zhang K, Bulkley D, Sun M, Smith AM, Rizo AN, Moss F, Brilot AF, Pourmal S, Trenker R, Pospiech T, Gupta S, Barsi-Rhyne B, Belyy V, Barile-Hill AW, Nock S, Liu Y, Krogan NJ, Ralston CY, Swaney DL, García-Sastre A, Ott M, Vignuzzi M, QCRG Structural Biology Consortium, Walter P, Manglik A. An ultra-potent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike. Science. 10.1126/science.abe3255, 2020
(PMID: 33154106)
Wang L, Walter P. Msp1/ATAD1 in Protein Quality Control and Regulation of Synaptic Activities. Annu. Rev. Cell Dev. 36:141–64, 2020
(PMID: 32886535)
Rabouw HH, Visser LJ, Passchier TC, Langereis MA, Liu F, Giansanti P, van Vliet ALW, Dekker JG, van der Grein SG, Saucedo JG, Anand AA, Trellet ME, Bonvin AMJJ, Walter P, Heck AJR, de Groot RJ, van Kuppeveld FJM. Inhibition of the integrated stress response by viral proteins that block p-eIF2–eIF2B association. Nat Microbiol 5(11):1361-1373, 2020
(PMID: 32690955)
Dufey E, Bravo-San Pedro JM, Eggers C, González-Quiroz M, Urra H, Sagredo AI, Sepulveda D, Pihán P, Carreras-Sureda A, Hazari Y, Sagredo EA, Gutierrez D, Valls C, Papaioannou A, Acosta-Alvear D, Campos G, Domingos PM, Pedeux R, Chevet E, Alvarez A, Godoy P, Walter P, Glavic A, Kroemer G, Hetz C. Genotoxic stress triggers the activation of IRE1α-dependent RNA decay to modulate the DNA damage response. Nat Commun 11:2401, 2020
(PMID: 32409639)
Carter SD, Hampton CM, Langlois R, Melero R, Farino ZJ, Calderon MJ, Li W, Wallace CT, Tran NH, Grassucci RA, Siegmund SE, Pemberton J, Morgenstern TJ, Eisenman L, Aguilar JI, Greenberg NL, Levy ES, Yi E, Mitchell WG, Rice WJ, Wigge C, Pilli J, George EW, Aslanoglou D, Courel M, Freyberg RJ, Javitch JA, Wills ZP, Area-Gomez E, Shiva S, Bartolini F, Volchuk A, Murray SA, Aridor M, Fish KN, Walter P, Balla T, Fass D, Wolf SG, Watkins SC, Carazo JM, Jensen GJ, Frank J, Freyberg Z. Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells. Sci Adv 6:eaay9572, 2020
(PMID: 32270040)
Wang L, Myasnikov A, Pan X, Walter P. Structure of the AAA protein Msp1 reveals mechanism of mislocalized membrane protein extraction. eLife 9:e54031, 2020
(PMID: 31999255)
Zhu PJ, Khatiwada S, Cui Y, Reineke LC, Dooling SW, Kim JJ, Li Wei, Walter P, Costa-Mattioli M. Activation of the ISR mediates the behavioral and neurophysiological abnormalities in Down syndrome. Science 366:843-849, 2019
(PMID: 31727829)
Harnoss JM, Le Thomas A, Shemorry A, Marsters SA, Lawrence DA, Lu M, Chen Y-C A, Qing J, Totpal K, Kan D, Segal E, Merchant M, Reichelt M, Ackerly Wallweber H, Wang W, Clark K, Kaufman S, Beresini MH, Laing ST, Sandoval W, Lorenzo M, Wu J, Ly J, De Bruyn T, Heidersbach A, Haley B, Gogineni A, Weimer RM, Lee D, Braun M-G, Rudolph J, VanWyngarden MJ, Sherbenou DW, Gomez-Bougie P, Amiot M, Acosta-Alvear D, Walter P, Ashkenazi A. Disruption of IRE1α through its kinase domain attenuates multiple myeloma. Proc Natl Acad Sci 116:16420-16429, 2019
(PMID: 31371506)
Kenner LR, Anand AA, Nguyen HC, Myasnikov AG, Klose CJ, McGeever LA, Tsai JC, Miller-Vedam LE, Walter P, Frost A. eIF2B-catalyzed nucleotide exchange and phosphoregulation by the integrated stress response. Science 364:491-495, 2019
(PMID: 31048491)
Rabouw HH, Langereis MA, Anand AA, Visser LJ, de Groot RJ, Walter P, van Kuppeveld FJM. The small molecule ISRIB suppresses the integrated stress response within a defined window of activation. Proc Natl Acad Sci 116:2097-2102, 2019
(PMID: 30674674)
Nguyen HG, Conn CS, Kye Y, Xue L, Forester CM, Cowan JE, Hsieh AC, Cunningham JT, Truillet C, Tameire F, Evans MJ, Evans CP, Yang JC, Hann B, Koumenis C, Walter P, Carroll PR, Ruggero D. Development of a stress response therapy targeting aggressive prostate cancer. Sci Transl Med 10:439, 2018
(PMID: 29720449)
Tsai JC, Miller-Vedam LE, Anand AA, Jaishankar P, Nguyen HC, Renslo AR, Frost A, Walter P. Structure of the nucleotide exchange factor eIF2B reveals mechanism of memory-enhancing molecule. Science 359:6383, 2018
(PMID: 29599213)
Karagöz GE, Acosta-Alvear D, Nguyen HT, Lee CP, Chu F, Walter P. An unfolded protein-induced conformational switch activates mammalian IRE1. eLife 6:e30700, 2017
(PMID: 28971800)
Placzek AN, Prisco GV, Khatiwada S, Sgritta M, Huang W, Krnjević K, Kaufman RJ, Dani JA, Walter P, Costa-Mattioli M. eIF2α-mediated translational control regulates the persistence of cocaine-induced LTP in midbrain dopamine neurons. eLife e17517, 2016
(PMID: 27960077)
Gallagher C, Garri C, Cain E, Ang K, Wilson C, Chen S, Hearn B, Jaishankar P, Aranda-Diaz A, Arkin M, Renslo A, Walter P. Ceapins are a new class of unfolded protein response inhibitors, selectively targeting the ATF6α branch. eLife 5:e11878, 2016
(PMID: 27435960)
Placzek AN, Molfese DL, Khatiwada S, Di Prisco GV, Huang W, Sidrauski C, Krnjević K, Amos CL, Ray R, Dani JA, Walter P, Salas R, Costa-Mattioli M. Translational control of nicotine-evoked synaptic potentiation in mice and neuronal responses in human smokers by eIF2α. eLife 5:e12056, 2016
(PMID: 26928076)
Huang W, Placzek AN, Di Prisco GV, Khatiwada S, Sidrauski C, Krnjević K, Walter P, Dani JA, Costa-Mattioli M. Translational control by eIF2α regulates adolescent vulnerability to the synaptic and 2 behavioral effects of cocaine. eLife 5:e12052, 2016
(PMID: 26928234)
Starck SR, Tsai JC, Chen K, Shodiya M, Wang L, Yahiro K, Martins-Green M, Shastri N, Walter P. Translation from the 5’ untranslated region shapes the integrated stress response. Science 351:6272, 2016
(PMID: 26823435)
Osman C, Noriega T, Okreglak V, Fung J, Walter P. Integrity of the yeast mitochondrial genome, but not its distribution and inheritance, relies on mitochondrial fission and fusion. Proc Nat Acad Sci 112:947-56, 2015
(PMID: 25730886) (PDF)
Lang AB, John Peter AT, Walter P, Kornmann B. ER-mitochondrial junctions can be bypassed by dominant mutations in the endosomal protein Vps13. J Cell Biol 210:883-90, 2015
(PMID: 26370498)
Sidrauski C, Tsai JC, Kampmann M, Hearn BR, Vedantham P, Jaishankar P, Sokabe M, Mendez A, Newton BW, Tang EL, Verschueren E, Johnson JR, Krogan NJ, Fraser CS, Weissman JS, Renslo AR, Walter P. Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response. eLife 4:e07314, 2015
(PMID: 25875391)
Sidrauski C, McGeachy A, Ingolia N, Walter P. The small molecule ISRIB reverses the effects of eIF2α phosphorylation on translation and stress granule assembly. eLife 4:e05033, 2015
(PMID: 25719440) (PDF)
Di Prisco G, Huang W, Buffington S, Hsu C, Bonnen P, Placzek A, Sidrauski C, Krnjević K, Kaufman R, Walter P, Costa-Mattioli M. Translational control of mGluR-dependent long-term depression and object-place learning by eIF2α. Nat Neurosci 17:1073-82, 2014
(PMID: 24974795)
Sidrauski C, Acosta-Alvear D, Khoutorsky A, Vedantham P, Hearn B, Li H, Gamache K, Gallagher C, Ang K, Wilson C, Okreglak V, Ashkenazi A, Hann B, Nader K, Arkin M, Renslo A, Sonenberg N, Walter P. Pharmacological brake-release of mRNA translation enhances cognitive memory. eLife 2:e00498, 2013
(PMID: 23741617)
Kimmig P, Diaz M, Zheng J, Williams C, Lang A, Aragón T, Li H, Walter P. The unfolded protein response in fission yeast modulates stability of select mRNAs to maintain protein homeostasis. eLife 1:e00048, 2012
(PMID: 23066505)
Nguyen T, Chandrasekar S, Neher S, Walter P, Shan S. Concerted complex assembly and GTPase activation in the chloroplast signal recognition particle. Biochemistry 50:7208-17, 2011
(PMID: 21780778)
Korennykh A, Egea P, Korostelev A, Finer-Moore J, Stroud R, Zhang C, Shokat K, Walter P. Cofactor-mediated conformational control in the bifunctional kinase/RNase Ire1. BMC Biol 9:48, 2011
(PMID: 21729334)
Korennykh A, Korostelev A, Egea P, Finer-Moore J, Stroud R, Zhang C, Shokat K, Walter P. Structural and functional basis for RNA cleavage by Ire1. BMC Biol 9:47, 2011
(PMID: 21729333)
Aguilar PS, Fröhlich F, Rehman M, Shales M, Ulitsky I, Olivera-Couto A, Braberg H, Shamir R, Walter P, Mann M, Ejsing CS, Krogan NJ, Walther TC. A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. Nat Struct Mol Biol 7:901-8, 2010
(PMID: 20526336)
Engel A, Aguilar PS, Walter P. The yeast cell fusion protein Prm1p requires covalent dimerization to promote membrane fusion. PLoS One 5:e10593, 2010
(PMID: 20485669)
Aguilar PS, Heiman MG, Walther TC, Engel A, Schwudke D, Gushwa N, Kurzchalia T, Walter P. Structure of sterol aliphatic chains affects yeast cell shape and cell fusion during mating. PNAS 9:4170-5, 2010
(PMID: 20150508)
Lin J, Li H, Zhang Y, Ron D, Walter P. Divergent effects of PERK and IRE1 signaling on cell viability. PLoS ONE 4:e4170, 2009
(PMID: 19137072)
Guo Y, Walther T, Rao M, Stuurman N, Goshima G, Terayama K, Wong J, Vale R, Walter P, Farese R. Functional genomic screen reveals genes involved in lipid-droplet formation and utilization. Nature 453:657-61, 2008
(PMID: 18408709)
Engel A, Walter P. Membrane lysis during biological membrane fusion: collateral damage by misregulated fusion machines. J Cell Biol 183:181-6, 2008
(PMID: 18852300)
Korennykh A, Egea P, Korostelev A, Finer-Moore J, Zhang C, Shokat K, Stroud R, Walter P. The unfolded protein response signals through high-order assembly of Ire1. Nature 457:687-93, 2008
(PMID: 19079236)
Walther T, Aguilar P, Fröhlich F, Chu F, Moreira K, Burlingame A, Walter P. Pkh-kinases control eisosome assembly and organization. EMBO J 26:4946-55, 2007
(PMID: 18034155)
Lin J, Li H, Yasumura D, Cohen H, Zhang C, Panning B, Shokat K, Lavail M, Walter P. IRE1 signaling affects cell fate during the unfolded protein response. Science 318:944-9, 2007
(PMID: 17991856)
Heiman M, Engel A, Walter P. The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating. J Cell Biol 176:209-22, 2007
(PMID: 17210951)
Aguilar P, Engel A, Walter P. The plasma membrane proteins Prm1 and Fig1 ascertain fidelity of membrane fusion during yeast mating. Mol Biol Cell 18:47-56, 2007
(PMID: 17151357)
Kaiser S, Brickner J, Reilein A, Fenn T, Walter P, Brunger A. Structural basis of FFAT motif-mediated ER targeting. Structure 13:1035-45, 2005
(PMID: 16004875)
Chu F, Shan S, Moustakas D, Alber F, Egea P, Stroud R, Walter P, Burlingame A. Unraveling the interface of signal recognition particle and its receptor by using chemical cross-linking and tandem mass spectrometry. Proc Natl Acad Sci 101:16454-9, 2004
(PMID: 15546976)
Papa F, Zhang C, Shokat K, Walter P. Bypassing a kinase activity with an ATP-competitive drug. Science 302:1533-7, 2003
(PMID: 14564015)
Helenius J, Ng D, Marolda C, Walter P, Valvano M, Aebi M. Translocation of lipid-linked oligosaccharides across the ER membrane requires Rft1 protein. Nature 415:447-50, 2002
(PMID: 11807558)
Dong B, Niwa M, Walter P, Silverman R. Basis for regulated RNA cleavage by functional analysis of RNase L and Ire1p. RNA 7:361-73, 2001
(PMID: 11333017)
Ng D, Spear E, Walter P. The unfolded protein response regulates multiple aspects of secretory and membrane protein biogenesis and endoplasmic reticulum quality control. J Cell Biol 150:77-88, 2000
(PMID: 10893258)
Kremerskothen J, Zopf D, Walter P, Cheng J, Nettermann M, Niewerth U, Maraia R, Brosius J. Heterodimer SRP9/14 is an integral part of the neural BC200 RNP in primate brain. Neurosci Lett 245:123-6, 1998
(PMID: 9605471)
Ng DTW & Walter P. ER membrane protein complex required for nuclear fusion. J Cell Biol 132:499-509, 1996
(PMID: not available)
Ng D, Brown J, Walter P. Signal sequences specify the targeting route to the endoplasmic reticulum membrane. J Cell Biol 134:269-78, 1996
(PMID: 8707814)
Ng D, Walter P. ER membrane protein complex required for nuclear fusion. J Cell Biol 132:499-509, 1996
(PMID: 8647883)
Young J, Ursini J, Legate K, Miller J, Walter P, Andrews D. An amino-terminal domain containing hydrophobic and hydrophilic sequences binds the signal recognition particle receptor alpha subunit to the beta subunit on the endoplasmic reticulum membrane. J Biol Chem 270:15650-7, 1995
(PMID: 7797564)
Brown JD, Ng DTW, Ogg SC, Walter P. Targeting pathways to the endoplasmic reticulum membrane. Cold Spring Harb Symp Quant Biol 60:23-30, 1995
(PMID: 8824374)
Brown J, Hann B, Medzihradszky K, Niwa M, Burlingame A, Walter P. Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression. EMBO J 13:4390-400, 1994
(PMID: 7925282)
Selinger D, Brennwald P, Althoff S, Reich C, Hann B, Walter P, Wise J. Genetic and biochemical analysis of the fission yeast ribonucleoprotein particle containing a homolog of Srp54p. Nucleic Acids Res 22:2557-67, 1994
(PMID: 8041618)
Green N, Fang H, Walter P. Mutants in three novel complementation groups inhibit membrane protein insertion into and soluble protein translocation across the endoplasmic reticulum membrane of Saccharomyces cerevisiae. J Cell Biol 116:597-604, 1992
(PMID: 1730771)
Hann B, Stirling C, Walter P. SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity. Nature 356:532-3, 1992
(PMID: 1313947)
Andrews D, Lauffer L, Walter P, Lingappa V. Evidence for a two-step mechanism involved in assembly of functional signal recognition particle receptor. J Cell Biol 108:797-810, 1989
(PMID: 2537835)
Lingelbach K, Zwieb C, Webb J, Marshallsay C, Hoben P, Walter P, Dobberstein B. Isolation and characterization of a cDNA clone encoding the 19 kDa protein of signal recognition particle (SRP): expression and binding to 7SL RNA. Nucleic Acids Res 16:9431-42, 1988
(PMID: 2460823)
Walter P, Lingappa V. Mechanism of protein translocation across the endoplasmic reticulum membrane. Annu Rev Cell Biol 2:499-516, 1986
(PMID: 3030381)
Müller M, Ibrahimi I, Chang C, Walter P, Blobel G. A bacterial secretory protein requires signal recognition particle for translocation across mammalian endoplasmic reticulum. J Biol Chem 257:11860-3, 1982
(PMID: 6749848)
Blobel G, Walter P, Chang C, Goldman B, Erickson A, Lingappa V. Translocation of proteins across membranes: the signal hypothesis and beyond. Symp Soc Exp Biol 33:9-36, 1979
(PMID: 524275)
Walter P, Jackson R, Marcus M, Lingappa V, Blobel G. Tryptic dissection and reconstitution of translocation activity for nascent presecretory proteins across microsomal membranes. Proc Natl Acad Sci 76:1795-9, 1979
(PMID: 109833)