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Synthesis in vitro and translocation of apolipoprotein AI across microsomal vesicles.
Stoffel W, Blobel G, Walter P. Synthesis in vitro and translocation of apolipoprotein AI across microsomal vesicles. Eur J Biochem 120:519-22, 1981 (PMID : 6174327) (PDF)
Abstract
Apolipoprotein AI of rats has been synthesized in a cell-free wheat germ system and cotranslationally translocated into dog pancreas microsomal vesicles. Translocation is accompanied by cleavage of a signal sequence of 18 amino acid residues and is dependent on the recently purified signal recognition protein.