Apolipoprotein AI of rats has been synthesized in a cell-free wheat germ system and cotranslationally translocated into dog pancreas microsomal vesicles. Translocation is accompanied by cleavage of a signal sequence of 18 amino acid residues and is dependent on the recently purified signal recognition protein.
Synthesis in vitro and translocation of apolipoprotein AI across microsomal vesicles.
Stoffel W, Blobel G, Walter P. Synthesis in vitro and translocation of apolipoprotein AI across microsomal vesicles. Eur J Biochem 120:519-22, 1981
(PMID : 6174327) (PDF)
(PMID : 6174327) (PDF)