Walter Lab

  • Walter Lab
  • Research
    • The Unfolded Protein Response and IRE1 Signaling in Health and Disease
    • Organellar quality control, dynamics, and inheritance
    • RNA processing in the unfolded protein response
    • The integrated stress response and its role in cognition
    • ATF6-branch signaling through regulated proteolysis
  • Lab Members
    • Current
    • Alumni
  • Contact Us
  • Social
  • Publications
  • News & Notes

Preparation of microsomal membranes for cotranslational protein translocation.

Walter P, Blobel G. Preparation of microsomal membranes for cotranslational protein translocation. Meth Enzymol 96:84-93, 1983
(PMID : 6656655) (PDF)

Abstract

Secretory, lysosomal, and many integral membrane proteins are translocated across or asymmetrically integrated into the membrane of the rough endoplasmic reticulum.l,la The events of this translocation or integration process can be faithfully reproduced in vitro. 2-4 For this purpose, an in vitro protein translation system programmed with a suitable mRNA is supplemented with microsomal membranes, a fraction of closed vesicles derived from the rough endoplasmic reticulum. 2 In all cases investigated, translocation is a cotranslational process; i.e., the nascent chain is vectorially translocated across the membrane as it emerges from the ribosome. Consequently, the microsomal membrane fraction has to be present during protein synthesis. This also implies that  all the components added to study the translocation process have to be compatible with in vitro protein synthesis.: If the microsomal vesicles are added after protein synthesis is completed, a post-translational translocation is not observed.


Deprecated: genesis_footer_creds_text is deprecated since version 3.1.0! Use genesis_pre_get_option_footer_text instead. This filter is no longer supported. You can now modify your footer text using the Theme Settings. in /nas/content/live/wlabdemo/wp-includes/functions.php on line 5698

Copyright © 2022  Walter Lab | University of California, San Francisco | Howard Hughes Medical Institute