Siegel V, Walter P. Functional dissection of the signal recognition particle. Trends Biochem Sci 13:314-6, 1988
(PMID: 2856455) (PDF)
(PMID: 2856455) (PDF)
Abstract
Biochemical mutagenesis – alteration or removal of specific domains within a biological structure -followed by functional analysis, gives insight into structure-function relationships. We describe the analysis of the signal recognition particle, a ribonucleoprotein known to be required for the entry of most proteins into the secretory pathway, as an example of the strength of this approach.